# Install packages
if (!requireNamespace("ggpubr", quietly = TRUE)) {
install.packages("ggpubr")
}
# Load packages
library(ggpubr)
Dotchart
Sliding bead chart is a graph of beads sliding on a column. It is the superposition of bar chart and scatter chart.
Setup
System Requirements: Cross-platform (Linux/MacOS/Windows)
Programming language: R
Dependent packages:
ggpubr
Data Preparation
The loaded data are gene names and their corresponding gene expression values and groups.
# Load data
<- read.delim("files/Hiplot/046-dotchart-data.txt", header = T)
data
# View data
head(data)
Name Value Group
1 BMP2 18.7 Group1
2 XIST 14.3 Group1
3 C19orf38 16.4 Group1
4 PDZD3 17.3 Group1
5 MAPRE2 15.2 Group1
6 IRF4 10.4 Group1
Visualization
# Dotchart
<- ggdotchart(data, x = "Name", y = "Value", group = "Group", color = "Group",
p rotate = T, sorting = "descending",
y.text.col = F, add = "segments", dot.size = 2) +
xlab("Name") +
ylab("Value") +
ggtitle("DotChart Plot") +
scale_color_manual(values = c("#e04d39","#5bbad6","#1e9f86")) +
theme_classic() +
theme(text = element_text(family = "Arial"),
plot.title = element_text(size = 12,hjust = 0.5),
axis.title = element_text(size = 12),
axis.text = element_text(size = 10),
axis.text.x = element_text(angle = 0, hjust = 0.5,vjust = 1),
legend.position = "right",
legend.direction = "vertical",
legend.title = element_text(size = 10),
legend.text = element_text(size = 10))
p

Each color represents a different grouping, so that the differences in gene expression values can be intuitively understood.